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http://nopr.niscpr.res.in/handle/123456789/44811| Title: | Standardization of protocol for genomic DNA isolation in Melia composita Benth. (S. M. dubia Cav.) |
| Authors: | Sharma, Shruti Kumar, Ashok Dobhal, Shivani Rawat, Anita |
| Keywords: | Melia composite;DNA extraction;Inter simple sequence repeat (ISSR);Simple sequence repeat (SSR) |
| Issue Date: | Jan-2018 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | A simple, rapid and efficient method for extraction of genomic DNA from young leaves of Melia composita Benth. (Syn. M dubia Cav.) was optimized for obtaining fine quality DNA. The yield of extracted DNA ranged from 118.40 to 3192.40 µg /500 mg of tissue with absorbance ratio (A260/A280) ranging from 1.41 to 1.96. The quality of extracted DNA was also evaluated by agarose gel electrophoresis which showed a single intact band. The isolated DNA was free of any contaminating proteins, polysaccharides and coloured pigments and successfully amplify inter simple sequence repeat (ISSR) and simple sequence repeat (SSR) markers. |
| Page(s): | 185-188 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Appears in Collections: | IJBT Vol.17(1) [January 2018] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 17(1) 185-188.pdf | 681.54 kB | Adobe PDF | View/Open |
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