Please use this identifier to cite or link to this item:
http://nopr.niscpr.res.in/handle/123456789/45112| Title: | Identification and Characterization of a Protease Encoding Metagenome Clone from Dairy Effluent |
| Authors: | Lal, R Soni, K B Alex, S Sreekantan, L Reghunath, B R |
| Keywords: | Metagenome;DNA;Transformed |
| Issue Date: | Oct-2018 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Metagenomic DNA was isolated from dairy effluent collected from a milk processing unit. The Hpa I digests of metagenome were cloned in pEZ BAC vector. The transformed DH5 colonies were selected by blue white screening on chloramphenicol (25μg/ml) containing plates. Functional screening of the library on skim milk agar showed a clone having protease activity. This clone was confirmed to have a metagenome insert of size approx. 9kb. The crude cell extract of this clone at 24th h of growth showed protease activity of 166.99U/ml (111.32U/mg protein). The protease was found to be alkaline and had maximum activity at 400C and pH 9.0. |
| Page(s): | 583-586 |
| ISSN: | 0975-1084 (Online); 0022-4456 (Print) |
| Appears in Collections: | JSIR Vol.77(10) [October 2018] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| JSIR 77(10) 583-586.pdf | 192.89 kB | Adobe PDF | View/Open |
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