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http://nopr.niscpr.res.in/handle/123456789/5141| Title: | Purification of phycobiliproteins from Nostoc muscorum |
| Authors: | Ranjitha, K Kaushik, B D |
| Keywords: | Nostoc muscorum;Phycobiliproteins;Phycoerythrin;Phycocyanin;Allophycocyanin |
| Issue Date: | May-2005 |
| Publisher: | CSIR |
| IPC Code: | C 12 N 1/12 |
| Abstract: | Purification of phycobiliproteins from phycoerythrin (PE) rich strain of Nostoc muscorum was carried out. Salting out with 55 % (NH4)2SO4 precipitates >80 % of each class of phycobiliprotein. Chromatography on DEAE cellulose-52 column gives pure PE (A562/A280 = 8.12), while rechromatography of the blue coloured fraction from this column using a hydroxy apatite column yields pure phycocyanin (PC) (A615/A280 = 3.89). SDS-PAGE analysis of purified PE and PC yields two polypeptides (~19.4 and 16.9 kDa). PE is found to be stable between pH 5-9. This protocol helps to get highly pure PE (72%) and PC (39%). |
| Page(s): | 372-375 |
| ISSN: | 0975-1084 (Online); 0022-4456 (Print) |
| Appears in Collections: | JSIR Vol.64(05) [May 2005] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| JSIR 64(5) 372-375.pdf | 209.47 kB | Adobe PDF | View/Open |
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