<i style="">In vitro</i> propagation of an endemic umbellifer, <i style="">Hydrocotyle conferta</i>

Abstract

A protocol for <i style="">in vitro </i>propagation of <i style="">Hydrocotyle conferta</i> Wight (Apiaceae) through axillary bud multiplication was established. Murashige and Skoog (MS) medium with 6.66 <img src='/image/spc_char/micro.gif'> <i style="">M</i> N⁶-benzyladenine (BA) and 5.37 <img src='/image/spc_char/micro.gif'> <i style="">M</i> <img src='/image/spc_char/alpha.gif'>-naphthalenacetic acid (NAA) was best suited for axillary bud multiplication including a mean of 21 shoots/node. Excision and culture of the nodal segments from the <i style="">in vitro</i> shoots on fresh medium with same concentrations of BA and NAA facilitated development of more than 25 shoots/node. Subsequent cultures enhanced the rate of shoot proliferation. The developed shoots rooted best on half strength MS medium with 0.54 <img src='/image/spc_char/micro.gif'> <i style="">M</i> NAA. Plantlets established in pots exhibited 95% survival.