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http://nopr.niscpr.res.in/handle/123456789/5532| Title: | In vitro propagation of an endemic umbellifer, Hydrocotyle conferta |
| Authors: | Karuppusamy, S Aruna, V Kiranmai, C Pullaiah, T |
| Keywords: | axillary bud multiplication;Hydrocotyle conferta;endemic umbellifer |
| Issue Date: | Oct-2007 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.8 A01H4/00 |
| Abstract: | A protocol for in vitro propagation of Hydrocotyle conferta Wight (Apiaceae) through axillary bud multiplication was established. Murashige and Skoog (MS) medium with 6.66  M N6-benzyladenine (BA) and 5.37 M  -naphthalenacetic acid (NAA) was best suited for axillary bud multiplication including a mean of 21 shoots/node. Excision and culture of the nodal segments from the in vitro shoots on fresh medium with same concentrations of BA and NAA facilitated development of more than 25 shoots/node. Subsequent cultures enhanced the rate of shoot proliferation. The developed shoots rooted best on half strength MS medium with 0.54 M NAA. Plantlets established in pots exhibited 95% survival. |
| Page(s): | 541-544 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Appears in Collections: | IJBT Vol.06(4) [October 2007] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 6(4) 541-544.pdf | 266.13 kB | Adobe PDF | View/Open |
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