Please use this identifier to cite or link to this item:
http://nopr.niscpr.res.in/handle/123456789/5853| Title: | In vitro cloning of ornamental species of Dianthus |
| Authors: | Pareek, Aparna Kantia, Archana Kothari, S L |
| Keywords: | Dianthus caryophyllus;Dianthus chinensis;Dianthus barbatus;shoot tip;nodal segments;shoot bud proliferation |
| Issue Date: | Apr-2004 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.7 A 01 H 4/00, 5/00 |
| Abstract: | Dianthus caryophyllus, D. chinensis and D. barbatus were micropropagated from shoot tips and nodal segments, which were cultured on MS medium supplemented with BAP and NAA. Best response in terms of multiple shoot formation was observed on MS medium with NAA (0.5 mg/l) + BAP (1 mg/l). In four weeks 10-15 shoots were formed. Every two weeks, inoculum of 6-8 shoots was transferred to a fresh medium for further shoot differentiation. Rooting of in vitro shoots was achieved on half strength, MS + IAA (0.5 mg/l). Plantlets that were transferred to pots matured and flowered and resembled seed grown plants Variation of any sort could be avoided as regeneration of shoots was obtained without intervening callusing. |
| Page(s): | 263-266 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Appears in Collections: | IJBT Vol.03(2) [April 2004] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 3(2) 263-266.pdf | 111.03 kB | Adobe PDF | View/Open |
Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.